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RNA transcripts, miRNA-sized fragments and proteins produced from D4Z4 units: new candidates for the pathophysiology of FSHD

Posted by Wan-Ching Wu on June 1, 2009

Deletion of a subset of the D4Z4 macrosatellite repeats in the subtelomeric region of chromosome 4q causes facioscapulohumeral muscular dystrophy (FSHD) when occurring on a specific haplotype of 4qter (4qA161).
 

Several genes have been examined as candidates for causing FSHD, including the DUX4 homeobox gene in the D4Z4 repeat, but none have been definitively shown to cause the disease, or has the full extent of transcripts from the D4Z4 region been carefully characterized.

Using strand-specific RT–PCR, we have identified several sense and antisense transcripts originating from the 4q D4Z4 units in wild-type and FSHD muscle cells.

Consistent with prior reports, we find that the DUX4 transcript from the last (most telomeric) D4Z4 unit is polyadenylated and has two introns in its 3-prime untranslated region.

In addition, we show that this transcript generates

  • (i) small si/miRNA-sized fragments,
  • (ii) uncapped, polyadenylated 3-prime fragments that encode the conserved C-terminal portion of DUX4 and
  • (iii) capped and polyadenylated mRNAs that contain the double-homeobox domain of DUX4 but splice-out the C-terminal portion.

Transfection studies demonstrate that translation initiation at an internal methionine can produce the C-terminal polypeptide and developmental studies show that this peptide inhibits myogenesis at a step between MyoD transcription and the activation of MyoD target genes.

Together, we have identified new sense and anti-sense RNA transcripts, novel mRNAs and mi/siRNA-sized RNA fragments generated from the D4Z4 units that are new candidates for the pathophysiology of FSHD.

 

Human Molecular Genetics 2009 18(13):2414-2430

Lauren Snider1,{dagger}, Amy Asawachaicharn1,{dagger}, Ashlee E. Tyler1, Linda N. Geng1, Lisa M. Petek2, Lisa Maves1, Daniel G. Miller2, Richard J.L.F. Lemmers4, Sara T. Winokur5, Rabi Tawil6, Silvère M. van der Maarel4, Galina N. Filippova1 and Stephen J. Tapscott1,3,*

1 Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA2 Department of Pediatrics 3 Department of Neurology, University of Washington, Seattle, WA 98195, USA4 Department of Human Genetics, Leiden University Medical Center, Leiden, The Netherlands5 Department of Biological Chemistry, University of California, Irvine, CA 92697, USA6 Department of Neurology, University of Rochester, Rochester, NY 14642, USA

*To whom correspondence should be addressed at: Fred Hutchinson Cancer Research Center, Mailstop C3-168, 1100 Fairview Ave North, Seattle, WA 98109, USA. Tel: +1 2066674499; Fax: +1 2066676524; Email: stapscot@fhcrc.org    Received January 5, 2009; Revised March 12, 2009; Accepted April 6, 2009

 

{dagger}The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors. Genbank sequence accession number: FJ439133.